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 หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Enzymes-Resistant Starch (RS III) from Pullulanase- Debranched High Amylose Rice Starch) ผู้เขียน: จิรภา พงษ์จันตา, อัญชนีย์ อุทัยพัฒนาชีพ,  ดร.อรอนงค์ นัยวิกุล, ศาสตราจารย์ , ดร.เกื้อกูล ปิยะจอมขวัญ สื่อสิ่งพิมพ์:pdf AbstractIn this study, a debranching enzyme (Pullulanase, EC. 232-983-9P, 8U/g starch at 55?C for 0 to 48 hr) was introduced to modify the amylopectin molecules of 15% (w/w) high amylose (32.10%) rice starches suspension which were gelatinized at 75?C for 30 min. The result showed that the retrogradation of debranched starches with different degree of hydrolysis were yielded 0.14 to 1.55% which each samples was then induced at 4?C for 16 hr. Afterward, the one freeze-thaw cycle process (-10/30?C) was applied to promote syneresis of retrograded starches. It was shown that pullulanase hydrolysis improved the degree of retrogradation from 28.10 to 54.53%. The resistant starch content of the RS III sample increased from 4.80 to 12.33% by 0 to 48 hr, respectively. Results showed that after debranching, starch molecule had rearranged and changed their crystal pattern from A to V-type by Xray diffraction analysis. The RS III sample formed a coarse honeycomb-like and filamentous network structure was observed with Scanning Electron Microscope. The estimated hydrolysis index and glycemic index of the RS III samples were between 35.61% to 62.70% and 59.29% to 73.47% respectively. |
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